The Gene Pulser Xcell™ is a modular electroporation system that includes a main unit, two accessory modules (the capacitance extender, or CE module, and . The Gene Pulser Xcell total system is the complete electroporation system for transfection of both eukaryotic and prokaryotic cells. The Gene Pulser Xcell total. We have one Year BIO-RAD Gene Pulser xCell Total Electroporation System for sale. Lab paid $ in Year but had to close.
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To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.
Gene Pulser Xcell™ Electroporation Systems | Life Science Research | Bio-Rad
Remove PBS; add sufficient trypsin to cover cells, and wait a few minutes to allow trypsin to detach cells. With over a decade of in The CE module is recommended for electroporation of most eukaryotic cells, including mammalian cells and plant protoplasts.
Write a Review All Questions. A choice of exponential or square waveforms. In these situations, the Certificate of Analysis is available for download, but other product details, such as the product name, are not.
Aspirate off cell culture media. We’ll be happy to assist you as soon as possible. Transfer the cells from each well to pre-warmed buffer in well plates. The PC module is recommended for the electroporation of bacteria and fungi, as well as other applications where high-voltage pulses are applied to samples of small volume and high resistance.
This page was last hene Why are Certificates of Analysis not on the Documents tab? Unable to load video.
Other buffers pylser be sufficient as well. After transfecting cells and allowing them to recover, analyze the transfection efficiency qualitatively, using epifluorescent microscopy, and quantitatively, using flow cytometry.
Gene Pulser Xcell Total System
Integral leads are included with the PC module. Remove cuvette from chamber.
The same field of view under epifluorescence shows a number of cells pulsdr expressing the GFP marker, but these are only a small percentage of the cells visible in the previous image. I cannot find the Certificate of Analysis I am looking for.
Gene Pulser: Analytical Instruments | eBay
Please try reloading the page. How do I apply for the education discount? The goal is to find the conditions which work best with your cells and your biological question. Gene Cloning and Analysis. The unit is responsive to the buttons. The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists like you.
If the problem continues, please let us know and we’ll try to help. Shown here are the percentages of GFP cells in the passage 13 cells which show a marked decrease in transfection percentage relative to the younger cells. Detailed parameters can be found for each of these cell lines, with details for the preparation of electrocompetent cells in the Gene Pulser Xcell system instructions or at www. With the cells passed 9 times prior to the electroporation, the overall pattern of transfection percentage is nearly identical, but with a very slight decrease in the transfection percentages.
Put cuvette in ShockPod chamber and deliver electric pulse. I am setting up an electroporation experiment using mouse primary MEF and my question is Flow cytometry can provide quantitative information about the transfection results under each of the different electroporation conditions. Manuals and Resources Note: Remove supernatant and resuspend cells in a known volume of PBS.
Gene Pulser Xcell to My Bench.